pbst wash removes protein ladder western blot

īurnette WN (1981) “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. YALOW RS, GLICK SM, ROTH J, BERSON SA (1964) Radioimmunoassay of human plasma ACTH. Sarge KD, Park-Sarge O-K (2009) Detection of proteins sumoylated in vivo and in vitro.

Biochim Biophys Acta, Mol Cell Res 1833:812–822 Voelkel T, Andresen C, Unger A et al (2013) Lysine methyltransferase Smyd2 regulates Hsp90-mediated protection of the sarcomeric titin springs and cardiac function. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times. Phototope®-HRP Western Blot Detection System: ( 7071 anti-rabbit) or ( 7072 anti-mouse) Includes biotinylated protein ladder, secondary ( 7074 anti. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Pere-Brissaud A, Blanchet X, Delourme D et al (2015) Expression of SERPINA3s in cattle: focus on bovSERPINA3-7 reveals specific involvement in skeletal muscle. Primary Antibody Dilution Buffer: 1X TBS, 0.1 Tween-20 with 5 BSA for 20 ml, add 2 ml 10X TBS to 18 ml water, mix. Proc Natl Acad Sci U S A 79:5249–5253Ĭonte A, Sigismund S (2017) Methods to investigate EGFR ubiquitination.

Wu WC, Walaas SI, Nairn AC, Greengard P (1982) Calcium/phospholipid regulates phosphorylation of a Mr “87k” substrate protein in brain synaptosomes.

Pbst wash removes protein ladder western blot